Abstract：

Objective: To assess effectiveness of intradermal injections of autologous fibroblasts for the treatment of facial rhytids and dermal depressions.

Design: Six-month prospective pilot study. Photographs and silicone molds were taken of a prominent rhytid or dermal depression from each patient prior to treatment and at 6 months after treatment.

Patients: Ten adults (age range, 24-69 years) who each exhibited a prominent rhytid or depressed facial scar.

Intervention: A 3-mm postauricular skin biopsy specimen from each participant was sent to Isolagen Technologies, Inc, laboratories, where a fibroblast cell line was developed. Three injection sessions were performed at 2-week intervals; target areas were the study site as well

as behind the ear.

Main Outcome Measures: Subjective improvement scores were obtained by each patient and 2 clinicians at every follow-up visit. Skin surface topographical features were evaluated with optical profilometry by comparing silicone molds before and after injection. Histological analysis was performed on a biopsy specimen of the postauricular injection site.

Results: Nine of 10 patients noted a 60% to 100% improvement with the treatment; clinicians made similar observations. Size reduction of 10% up to 85% of the study site was demonstrated by optical profilometry for every patient. Microscopically, there was evidence of increased thickness and density of dermal-layer collagen.

Conclusions: Intradermal injection of autologous fibroblasts may be an effective treatment option for facial rhytids and depressed scars.

摘要：

目的：评价皮内注射自体成纤维细胞治疗面部皱纹和皮肤凹陷的疗效。

设计：为期6个月的试验研究。在治疗前和治疗后6个月，对每名患者的面部皱纹或皮肤凹陷进行拍照和硅胶铸模。

患者：10名成年人(年龄24-69岁)，每个人都有明显的皱纹或面部凹陷疤痕。

干预：取每名参与者耳后皮肤活检标本3毫米，送至Isolagen Technologies，Inc.实验室，构建成纤维细胞系。每两周进行三次注射，注射区域也是研究位置（耳后）。

主要成果指标：来自每次随访时每位患者和2位临床医生的主观改善评分。通过比较注射前后的硅胶模具，用光学轮廓测定法评价皮肤表面形貌特征。对耳后注射部位的活检标本进行组织学分析。

结果：10名患者中有9名表示，在治疗后达到60%到100%的改善，与临床医生的观察结论类似。通过光学轮廓测量，每个患者的研究部位缩小了10%至85%。显微镜观察表明真皮层胶原的厚度和密度增加。

结论：皮内注射自体成纤维细胞可能是治疗面部皱纹和凹陷性疤痕的有效方法。